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iupac inner filter effect i03047.
the inner filter effects change the spectrum and intensity of the emitted light and they must therefore be considered when analysing the emission spectrum of fluorescent light.
the inner filter effect is a problem for any fluorescence.
the inner filter effect is a common problem in fluorescence spectroscopy affecting spectral measurements in particular in highly concentrated solutions the excitation beam is attenuated by the sample so that only the surface facing the excitation beam fluoresces strongly the centre of the cuvette which is imaged by the emission monochromator in.
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24 02 2014 inner filter effect https doi org 10 1351 goldbook i03047 in an emission experiment an apparent decrease in emission quantum yield and or distortion of bandshape as a result of reabsorption of emitted radiation during a light irradiation.
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the inner filter effect can be a problem for any fluorescence measurementnespecially where an absorbing component is being titrated into the cuvette the fluorescence is proportional to the light absorbed but absorbing light at one end of the cuvette means that it isn t available at the other end.
interquartile range wikipedia.
of a fluorophore called the inner filter effect ife 3 this well known ife depends on sample absorption and on instrument geometry and is usually significant even in samples with rather low absorption the error is about 8 at an absorbance of 0 06 in a 1 cm square cell 4 we have recently shown that ife correction is very useful.
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this effect is thought to occur due to filtering within the cochlea the hearing organ in the inner ear a complex sound is split into different frequency components and these components cause a peak in the pattern of vibration at a specific place on the cilia inside the basilar membrane within the cochlea.
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unsourced material may be challenged and removed in descriptive statistics the interquartile range iqr also called the midspread middle 50 or h spread is a measure of statistical dispersion being equal to the difference between 75th and 25th percentiles or between upper and lower quartiles iqr q3 q1.
inner filter effect wikipedia
